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Image Search Results
Journal: Analytical Chemistry
Article Title: Label-Free Quantitation of a Cancer Biomarker in Complex Media Using Silicon Photonic Microring Resonators
doi: 10.1021/ac902006p
Figure Lengend Snippet: Figure 6. Real-time, label-free detection of CEA using microring resonators. (A) Overlay of three time-resolved association curves for the same ring at each concentration of CEA. The colored traces are tangent lines to the association curve at t ) 0 and are used to determine the initial slope of sensor response. (B) Concentration-response calibration plot of the initial slope of sensor response versus CEA concentration upon introduction of antigen standard solutions. The dashed box in the corner of the graph represents the range shown in panel (C) for measurement of CEA concentrations of unknown samples. (C) Overlay of the unknown solutions on a concentration-response calibration plot for CEA as determined by the initial slope method covering a dynamic range comparable to a commercial ELISA.
Article Snippet:
Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay
Journal: Analytical Chemistry
Article Title: Label-Free Quantitation of a Cancer Biomarker in Complex Media Using Silicon Photonic Microring Resonators
doi: 10.1021/ac902006p
Figure Lengend Snippet: Figure 7. (A) Example sensor response following addition of CEA in 100% FBS. Initial slope is determined by the use of a linear fit of the baseline to subtract the drifting baseline from the change in signal caused by addition of CEA in FBS. (B) Overlay of the unknown solution on the concentration-response calibration plot for CEA in 100% FBS. The concentration was determined to be 61 ( 23 ng/mL, which is in good agreement with a commercial ELISA assay.
Article Snippet:
Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay