Journal: Biosensors & bioelectronics

Article Title: Microfluidic-based exosome isolation and highly sensitive aptamer exosome membrane protein detection for lung cancer diagnosis.

doi: 10.1016/j.bios.2022.114487

Figure Lengend Snippet: Fig. 6. Five key lung cancer protein biomarkers (PD-L1, CA125, CD63, CEA and EpCAM) were immunohistochemically stained in tumour tissues (A). The abun dances of the five biomarkers in each patient detected by Exo-AFS (B) and ELISA (C) (n = 3 independent experiments, the data are presented as mean value ± SD).

Article Snippet: Other ELISA kits were used in this study included human CD63 kit (Elabscience, E-EL-H2428c), human CA125 kit (Elabscience, E-EL-H0636c), human EpCAM kit (Elabscience, E-ELH1880c) and human CEA kit (Elabscience, E-EL-H6047).

Techniques: Staining, Enzyme-linked Immunosorbent Assay

Journal: Biosensors & bioelectronics

Article Title: Microfluidic-based exosome isolation and highly sensitive aptamer exosome membrane protein detection for lung cancer diagnosis.

doi: 10.1016/j.bios.2022.114487

Figure Lengend Snippet: Fig. 7. Exosome profiling for lung cancer detection. Five key protein markers (PD-L1, CA125, CD63, CEA and EpCAM) of lung cancer were assessed in exosome samples from 30 patients with lung cancer and 15 healthy controls (SUM: PD-L1+CA125+CEA). A, Exo-AFS (Top) and ELISA (Bottom) analyses for lung cancer diagnosis. Figure B–H are displayed in the format of scatterplot. B, Plasma PD-L1 levels detected by ELISA. C, Exosomal PD-L1 levels detected by ELISA. D, Exosomal PD-L1 levels detected by Exo-AFS. E, The exosomal PD-L1 levels detected by Exo-AFS and ELISA in the cohort. F, Exo-AFS analysis of four lung cancer biomarkers (CA125,CD63,CEA and EpCAM). G, ELISA analysis of the four biomarkers. H, ROC curves of all biomarkers detected in Exo-AFS and ELISA. I, The correlations of SUM signatures in Exo-AFS and ELISA for cancer diagnosis. The vertical line represents the detection threshold of ELISA (0.8640), the horizontal line represents the detection threshold of Exo-AFS (1938). Detailed statistics are listed in Table 1 (n = 3 independent experiments, the data are presented as mean value ± SEM, unpaired two-sided t-test, the detection threshold is calculated from the ROC curves with the optimal sensitivity and specificity. P values are indicated on the chart).

Article Snippet: Other ELISA kits were used in this study included human CD63 kit (Elabscience, E-EL-H2428c), human CA125 kit (Elabscience, E-EL-H0636c), human EpCAM kit (Elabscience, E-ELH1880c) and human CEA kit (Elabscience, E-EL-H6047).

Techniques: Enzyme-linked Immunosorbent Assay, Biomarker Discovery, Clinical Proteomics

Journal: Cancer Nanotechnology

Article Title: Artemisinin-loaded polylactic acid nanoparticles alleviate 1.2 N,N-dimethylhydrazine-induced colorectal cancer in Albino rats

doi: 10.1186/s12645-025-00320-7

Figure Lengend Snippet: Fig. 3 Effect of artemisinin (ART) and artemisinin-loaded polylactic acid nanoparticles (ART-PLA NPs) on serum levels of the tumor marker carcinoembryonic antigen (CEA) in the studied groups. Data are shown as mean ± SEM. Statistically significant differences were observed by one-way ANOVA followed by Tukey’s multiple comparisons test (***p < 0.001, **p < 0.01, *p < 0.05, ns = not significant)

Article Snippet: Nuclear factor kappa B (NF-kB-p65) (Cat. No. E-EL-R0674) and Carcinoembryonic Antigen (CEA) (Cat. No. E-EL-R0150) were purchased from Elabscience® (USA). cDNA synthesis kit (SensiFASTTM cDNA Synthesis Kit, Catalog number: BIO-65053) and SYBR Green Master Mix (2 × SensiFastTM SYBR) were purchased from Bioline (USA).

Techniques: Marker

Journal: Analytical Chemistry

Article Title: Label-Free Quantitation of a Cancer Biomarker in Complex Media Using Silicon Photonic Microring Resonators

doi: 10.1021/ac902006p

Figure Lengend Snippet: Figure 6. Real-time, label-free detection of CEA using microring resonators. (A) Overlay of three time-resolved association curves for the same ring at each concentration of CEA. The colored traces are tangent lines to the association curve at t ) 0 and are used to determine the initial slope of sensor response. (B) Concentration-response calibration plot of the initial slope of sensor response versus CEA concentration upon introduction of antigen standard solutions. The dashed box in the corner of the graph represents the range shown in panel (C) for measurement of CEA concentrations of unknown samples. (C) Overlay of the unknown solutions on a concentration-response calibration plot for CEA as determined by the initial slope method covering a dynamic range comparable to a commercial ELISA.

Article Snippet: CEA ELISA kits were purchased from GenWay Biotech (San Diego, CA) and Signosis (Sunnyvale, CA).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Analytical Chemistry

Article Title: Label-Free Quantitation of a Cancer Biomarker in Complex Media Using Silicon Photonic Microring Resonators

doi: 10.1021/ac902006p

Figure Lengend Snippet: Figure 7. (A) Example sensor response following addition of CEA in 100% FBS. Initial slope is determined by the use of a linear fit of the baseline to subtract the drifting baseline from the change in signal caused by addition of CEA in FBS. (B) Overlay of the unknown solution on the concentration-response calibration plot for CEA in 100% FBS. The concentration was determined to be 61 ( 23 ng/mL, which is in good agreement with a commercial ELISA assay.

Article Snippet: CEA ELISA kits were purchased from GenWay Biotech (San Diego, CA) and Signosis (Sunnyvale, CA).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Proteomic analysis of serum extracellular vesicles from biliary tract infection patients to identify novel biomarkers

doi: 10.1038/s41598-024-56036-y

Figure Lengend Snippet: Validation of CEACAM1 and CRB3 expression in serum EVs. ( A ) CEACAM1 and CRB3 were detectable by Western blot (WB) analysis (N total = 8). ( B ) Densitometric quantification of CEACAM1. ( C ) Densitometric quantification of CRB3. ( D ) CRB3 was detectable by ELISA analysis. ( E ) CEACAM1 was detectable by ELISA analysis. * p < 0.05.

Article Snippet: Protein levels were determined using ELISA kits according to the manufacturer’s instructions: Human CEACAM1 ELISA Kit (EK1361, BOSTER, China) and Human Protein Crumbs Homolog 3 (CRB3) ELISA Kit (abx386665, abbexa, UK).

Techniques: Biomarker Discovery, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay